Ministry of higher education and scientific research
University of Kufa /College of sciences
A Study of some side effects of
Bacillus thuringiensis on white rabbit males, and it's
antagonistic ability of Pseudomonas aeruginosa
in vivo and in vitro
A thesis
being submitted to the collage of science-University
of Kufa in partial fulfillment of the requirements for the degree of
Master of Science in Biology-
Microbiology
By
Jamal Muhammad Radha Ali Al-Shara
B.Sc.BIOLOGY
March 2006
Abstract
The mean aims of this study is to evaluate if there is any associated hazards with the biological formulation
" Thurin w.p " which is composed of an inoculum of the bacteria , B.thuringiensis and CaCO3 to
study the effect of different physical factors on the growth of this bacteria along with studying of it's
antagonistic effect on the growth of P.aeruginosa ,and it's pathogenicity on white rabbit males .
The results showed that there was no any cases of bacteremia and or septicemia associated with inoculated
rabbits , however there were some effects on some the hematological standards of the tested animals which
could be attributed to the associated carrier substance (calcium carbonate). There were no histopathological
effects on the internal viscera of the lab. Animals like liver , kidney , small intestine ,spleen …etc.
When the effects of some physical factors on the growth of B.thuringiensis is taken in consideration ,it was
shown that this bacteria can grow on awide scale of temperatures ranging from( 10 - 45Cú ) with
an optimal growth temperature 30 Cú . The bacteria also has an ability to grow on a wide range of sodium
chloride concentrations (1 - 10 %) ,but the growth was suppressed totally at 11% concentration .
On other hand , it was shown that B.thuringiensis had an ability to inhibited the growth of P.aeruginosa on the
culture medium, in a degree which is more or less the same as the suppressive effect of the antibiotics ,
Tobramicin and Norfloxacin . The study also demonstrated that P.aeruginosa showed antibiotic
resistance to thirteen antibiotic preparations .
The results justified the ability of the bacteria B.thuringiensis to confer a protection for the lab animal when given
in a triple dose each of (2 × 108 bacteria / ml) daily for seven days by it's ability to keep the hematological
parameters within the acceptable physiological limits on the histology of liver ,small intestine , spleen, kidney .
These parameters of blood and the internal organs were highly affected ,with a wide deviate from
the normal ones ,when the animal were treated by P.aeruginosa alone .
